In-vitro antimicrobial evaluation of Endodontic cavity sealers against Enterococcus faecalis

The aim of the present investigation was to evaluate the ability of four root canal sealers against the more resistive Enterococcus faecalis. E faecalis is a common pathogen which is a causative agent of oral cavities. The bacteriostatic effect was studied against the test organism with AH plus, Endorez, Metaplex and Grossmans sealer in 96 well titre plate. After different settling time (20 minutes, 1 day, 3days, and 7 days), the sealers were mixed with E.faecalis and incubated at humid environment. After incubation the mixer was diluted with 10-fold of tryptic soy broth and plated onto tryptic soy agar (TSA). The total number of colony-forming units permilliliter (CFU/mL) was determined. The maximum inhibition was observed with Grossman’s sealers after 20 minutes of settling time followed by AH plus sealer after 1 day settling time period. The Endorez and Metapex have showed the maximum growth inhibition after 1 day and 7 days settling period. Thus these results conclude that Metapex and Endorez is effective against E. faecalis, despite its slower action compared with the Grossman and AH plus sealers